Fudan University

报告摘要：

Live-cell fluorescence nanoscopy has revolutionized the study of cellular biology at the molecular level. However, the limited availability of appropriate fluorophores, which often exhibit poor cell permeability and produce nonspecific background signals, has hampered the widespread use of this technique. We present a strategy to convert rhodamines’ carboxyl group into an electron-deficient amide, preserving spectral properties while tuning the equilibrium between a fluorescent zwitterion and a non-fluorescent, cell-permeable spirolactam. Binding to cellular targets shifts the equilibrium toward the fluorescent form, yielding up to 1,000-fold signal enhancement. These cell-permeable, fluorogenic probes, spanning colors from green to near-infrared, enable wash-free super-resolution imaging of microtubules, F-actin, HaloTag, SNAP-tag, DNA, RNA, and lysosomes in live cells. To address photobleaching in covalently tethered protein tags, we engineered a regenerative TMP-tag (ReTMP; L28Q, R44L, H45Q) with highly fluorogenic ligands, enabling rapid reversible labeling within 1 min and ~90% fluorescence recovery after five bleach cycles. Optimized ligands achieved a 212-fold signal-to-noise ratio in wash-free live-cell imaging. This system supports >300-frame STED imaging of mitochondrial TOM20 at 80 nm resolution over 100 min and enhances long-term single-molecule tracking of membrane proteins, enabling extended, high-contrast visualization of cellular dynamics.

报告人简介：

Dr. Lu Wang got his Bachelor degree in China Pharmaceutical University (2006-2010) and Master degree in Fudan University (2010-2013). From 2013 to 2017, Dr. Lu Wang finished his Ph. D. program in National University of Singapore under the supervision of Prof. Young-tae Chang. Later, he started his post-doc research work with the support from Humboldt Fellowship in Max-Planck Institute under the guidance of Prof. Kai Johnsson. Since June 2021, Dr. Lu Wang joined the School of Pharmacy, Fudan University as a youth principle investigator with the support from the Overseas Young High-Level Talent Recruitment Programs. His research interests mainly focus on the molecular imaging and chemical biology, especially on the design and synthesis of fluorescent probes for bioimaging and biosensing. Till now, he has published more than 30 SCI papers in top journals, including Nat. Chem., Nat. Comm. J. Am. Chem. Soc, Angew. Chem. (total citation: 3700, h-index: 26).

报告地点：中国科学院物理研究所A楼BII段412会议室

邀请人：李迪 (lidi@iphy.ac.cn)